![]() ![]() Also, no dressing changes were needed in the areas covered with NTFS, due to good adherence of the biomaterial to the wound bed, suggesting its potential in reducing burn patient pain, hospital costs and overall workload of the health care team. The periods of 12 and 17 days required, respectively, for complete reepithelialization of this patient’s wounds in the right upper limb and the left upper limb, suggest effectiveness of NTFS as a xenograft for the treatment of human burns. Healing is expected within 2 weeks for SPTB and usually takes longer than 3 weeks for DPTB. A recent study showed tilapia collagen significantly induces epidermal growth factor and fibroblast growth factor expression, which can promote proliferation and differentiation of fibroblasts and keratinocytes. ![]() ![]() Another study found promising results with the application of marine collagen peptides from tilapia skin on deep partial-thickness scalds in rabbits. Tilapia collagen nano-fibers were also found to enhance skin wound healing speed in rats by promoting cell adhesion, proliferation and differentiation. When used as a xenograft for treatment of experimental burns in rats, NTFS led to improvements in the healing process and no relevant changes in hematological and biochemical parameters. NTFS can become the first nationally studied animal skin registered by ANVISA for use in burn treatment. Porcine skin was neither registered nationally for the use in burn wounds and has very low availability in the specialized centers. Although frog skin was previously used as a burn treatment in Brazil, it was never registered by the National Sanitary Surveillance Agency (ANVISA). Since allografts and xenografts appear to be equally effective, xenografts might be a superior choice for their increased safety and reduced price. Finally, firm coverage of the wounds with gauze and bandage was performed. Due to the still experimental nature of the treatment with NTFS, the researchers decided to apply silver sulfadiazine cream 1%, still used as standard treatment by almost all Brazilian burn centers, to the rest of the burned areas. (Fig.4) 4) Coverage of at least 1 cm of healthy skin in the borders of the wound and superposition of at least 1 cm between NTFS pieces are needed, both to ensure that eventual movement in the first days of treatment will not lead to uncovering of any area of the burn. (Fig.3) 3) the biomaterial was applied to the upper limbs of the patient. After cleaning the lesion with tap water and 2% chlorhexidine gluconate and removing necrotic and fibrinous tissue, (Fig. The patient was submitted to anesthesia and analgesia with 150 mg of ketamine, 10 mg of midazolam and 200 mg of tramadol. , apart from describing the steps of NTFS preparation, showed the biomaterial did not present variations in its microscopic and tensiometric structure after chemical sterilization and irradiation and recovered its natural consistency after the rehydration process. Prior to its use in the patient, the skin was washed in sterile 0.9% saline for 5 minutes, with this process being repeated three times in a row. NTFS was subjected to a rigorous process of chemical sterilization, glycerolization and irradiation, followed by microbiological tests for bacteria and fungi, before storage in refrigerated sterile packaging. Deep partial thickness burn in the left upper limb, after cleaning of the lesion. ![]()
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